This work was supported in parts by the Guangdong Provincial Key S&T Program (2018B030336001) and the Sanming Project of Medicine in Shenzhen (SZSM201611090). 4). in this study, the electron microscopy images of oil bodies were Some acyl-CoA enter the phosphatidylcholine pool, whereas other acyl-CoA are combined with glycerol-3-phosphate to form TAG. The 33 functional groups in the biological process, cellular component, and molecular function categories were classified according to the GO annotations of the DEGs. The four comparisons with the 85 DAF sample were grouped into two main clusters based on the patterns of differential gene expressions: 85 to 100 + 100 to 115 and 115 to 145 + 145 to 180 DAF (Supplemental Fig.

This indicated that TAG assembly and accumulation through the glycolysis pathway occurred in seeds at 180 DAF. FAD2 and FAD3 exhibited higher expressions than the other FAD genes (Figs. GC (Gas Chromatography) analysis revealed that the oil contained approximately 6.22% nervonic acid. (2019). Specifically, 754 DEGs were associated with FA metabolism, 34 were associated with arachidonic acid metabolism, 34 were associated with UFA biosynthesis, 42 were associated with cutin, suberin, and wax biosynthesis, 44 were associated with FA biosynthesis, 42 were associated with FA degradation, 45 were associated with FA elongation, and 20 were associated with linoleic acid metabolism. BDNF-TrkB signaling is involved in the histopathological damage, synaptic protein loss and inflammatory response caused by an electromagnetic pulse in rat brain cortex. Perspect. Web Close. The hallmarks of aging. (A) Heat map showing relative expression levels of three individuals of Lishui provenance at different seed development stages. Methods 12 357 360 doi: 10.1038/nmeth.3317, Kirk, R.E. You can easily wholesale quality seed extract well at wholesale prices on Made-in-China.com. & Salzberg, S.L. Advance the re-growth of cranial nerve. In the next step, we will use BDNF receptor inhibitor K252a to inactivate TrkB, and then investigate whether acer truncatum seed oil treatment still has the improved effects on aging mice. All the animals were provided with adequate food and water, and kept in a standard environment with temperature (22 2C) and a 12 h light-dark cycle. extract acer truncatum seed

Pharmacol. Inflammaging and Anti-Inflammaging: the Role of Cytokines in Extreme Longevity. Biochem. The datasets presented in this study can be found in online repositories. 9:680386. doi: 10.3389/fcell.2021.680386. Aging brain: the effect of combined cognitive and physical training on cognition as compared to cognitive and physical training alone - a systematic review. Dis. Urban forests, which are highly valuable for the urban environment, include trees from city streets that might be susceptible to wood-decay fungi. 1B). Neurobiol. Based on the qRT-PCR and RNA-seq analyses, FAD2/6 were highly expressed and FAD3/7/8 showed low expression. Rev. However, the lack of a complete genome sequence has limited both basic and applied research on A. truncatum.We describe a high-quality draft genome assembly comprising 633.28 Mb (contig N50 = Chronic nicotinamide riboside supplementation is well-tolerated and elevates NAD(+) in healthy middle-aged and older adults. Aging and age-related diseases share basic mechanisms largely involving in the inflammation (Ferrucci and Fabbri, 2018). Cell 153, 11941217.

Proteomic Profiling of Brain and Testis Reveals the Diverse Changes in Ribosomal Proteins in fmr1 Knockout Mice. Compared with young control, the mRNA levels of inflammatory factor including IL-1, TNF-, and IL-6 were significantly upregulated in the aging control mice, while acer truncatum seed oil markedly reversed the decreased mRNA levels of these pro-inflammatory factors in aging mice (Figures 6AC). Figure 5. Discov. Betaine attenuates Alzheimer-like pathological changes and memory deficits induced by homocysteine. Aging 13, 12671301. To validate the reliability of the RNA-seq results, 16 genes related to FA biosynthesis were selected for qRT-PCR validation (Fig. (2019). Prog. Docosahexaenoic acid dietary supplementation enhances the effects of exercise on synaptic plasticity and cognition.

The mice hippocampal tissues were homogenized, sonicated, and then centrifuged at 12,000 g for 30 min at 4C. WebAcer truncatum Bunge seed oil (ASO) is rich in -9 (53.93%) and -6 (30.7%) fatty acids (FAs) and characterized by 3-7% nervonic acid (NA, C24:1-9). These results suggest that BDNF signaling pathway may play an important role in the mechanisms of the oil administration improving learning and memory in aging mice. 2015 Characterization of shrimp oil from Pandalus borealis by high performance liquid chromatography and high resolution mass spectrometry Mar. During this study, we obtained comprehensive genomic data characterizing gene expressions at six time points of seed development in the A. truncatum tree. doi: 10.1080/1028415X.2020.1831262, Li, Q., Chen, J., Yu, X., and Gao, J. M. (2019). Fan et al. Biophys. Furthermore, they are primarily responsible for the (18:3) content of seeds (Hernndez et al., 2016). Aging, characterized by a time-dependent functional decline of physiological integrity (Lopez-Otin et al., 2013), is a major independent risk factor for many neurodegeneration diseases such as Alzheimers disease (AD), Parkinsons disease, Amyotrophic lateral sclerosis, and so on. The aging hippocampus: interactions between exercise, depression, and BDNF. WebThe manuscript ''Effectively improvement of the oxidative stability of Acer truncatum Bunge seed oil, a new woody oil food resource by rosemary extract'' investigated the effects of rosemary extract on the oxidation stability of the Acer truncatum seed oil. Inflammaging: disturbed interplay between autophagy and inflammasomes. (A) Oil treatment improved learning ability of aging mice shown by decreased escape latency during training phase detected by Morris water maze. 3 and 4), suggesting that they have an important role in UFA biosynthesis. (Zoonyms of Pallas). XH analyzed the data and performed the statistics. doi: 10.1038/nrd4024, Lopez-Otin, C., Blasco, M. A., Partridge, L., Serrano, M., and Kroemer, G. (2013). All data were expressed as mean SEM, n = 3 mice for each group. doi: 10.1002/mnfr.200900439, Azman, K. F., and Zakaria, R. (2019). Three biological replicates (nested with three technical replicates) are represented by the error bars (P < 0.05). 26 1595 1611 doi: 10.1007/s10068-017-0218-8, Crowe, A.J., Abenes, M., Plant, A. All data were expressed as mean SEM, n = 79 mice for each group. The Biology of Aging and Cancer: frailty. The ABI3 could promote oleosin gene expression in B. napus (Crowe et al., 2000). Compared with 2-month-old (2M-Ctrl) mice, the protein levels of BDNF, p-TrkB, p-ERK, p-Akt, and p-CREB decreased in 20-month-old (20M-Ctrl) mice, were reversed by oil treatment, which measured by western blotting (A), and quantitative analyses (BF). (2016). The activity of PI3K/Akt signaling influencing neuronal survival and synaptic plasticity was declined in the aging rats of brain (van der Heide et al., 2006; Jiang et al., 2013). J. Mol. Quantitative real-time polymerase chain reaction (qRT-PCR) validation of fusca3 (FUS3), leafy cotyledon1 (LEC1), leafy cotyledon1 (LEC1.1), Abscisic acid insensitive3 (ABI3), and wrinkled1 (WRI1) at five time points of Acer truncatum seed development. WebThe manuscript ''Effectively improvement of the oxidative stability of Acer truncatum Bunge seed oil, a new woody oil food resource by rosemary extract'' investigated the effects of rosemary extract on the oxidation stability of the Acer truncatum seed oil. Specific primers were designed with Primer5 (Supplemental Table 1). The results showed that their expression tendencies were consistent with the RNA-seq results.

In general, CREB/BDNF pathway may underlie the acer truncatum seed oil elevated expression of synaptic proteins in the aging mice to alleviate cognitive impairment. 112 301 307 doi: 10.1034/j.1399-3054.2001.1120301.x, Gao, J. 8023, revised 1978) and were approved by the Ethics Committee of the Shenzhen Center for Disease Control and Prevention. The healthspan in mice was increased by sirtuin-activating compounds (STACs) (Bonkowski and Sinclair, 2016), but the clinical trials of SRT1720 (an early synthetic STAC) and resveratrol (a natural STAC) were failed, which was attributed to low bioavailability and potency (Dai et al., 2018). MCODE analysis indicated the DE proteins in the oil treated aging mice were related to complement and coagulation cascades, inflammatory response and innate immune response. 2016 iTAK: A program for genome-wide prediction and classification of plant transcription factors, transcriptional regulators, and protein kinases Mol. We also found that supplementation of folate/vitamin B12 or betaine significantly reversed the memory deficits of a chronic hyperhomocysteinemia model or aged rats, respectively (Wei et al., 2011; Chai et al., 2013). 2006 Fisher, Ronald Alymer, p. 354. Small-molecule modulation of neurotrophin receptors: a strategy for the treatment of neurological disease. Regulation of FA biosynthesis has been shown to occur at multiple levels, among which transcriptional regulation is considered a major means of regulating the pathway (Millar et al., 2000). 2017 Bioconversion of conjugated linoleic acid by Lactobacillus plantarum CGMCC8198 supplemented with Acer truncatum Bunge seeds oil Food Sci. The average ratio of clean reads to raw reads was 97.27%. We used RStudio software to complete the omics heat map drawing and cluster analysis.

Evol.

For the sake of minimizing animal consumption and the importance of NA for human health, extraction of NA from plants has become a research hotspot. We here treated normal aging mice with acer truncatum seed oil, and found that the seed oil significantly improved the learning and memory ability. & Samaha, R.R. 2A), including two FAD2, one FAD3, one FAD5, one FAD6, and one FAD7/8 genes based on the phylogenetic analysis of A. thaliana. The oil content ranged from 17.81% to 36.56% (mean: 28.57%), which mainly consisted of 14 types of fatty acids. Total RNA was extracted from hippocampus using Trizol (Invitrogen, 15596026, United States). doi: 10.1038/297681a0, Moskalev, A. 7:a021758. The clean reads were submitted to the Sequence Read Archive (SRA) under study accession number SRS5367121 and biosample accession number SAMN12389479. 301:125286. doi: 10.1016/j.foodchem.2019.125286, Longo, F. M., and Massa, S. M. (2013). ACS Chem. (C) The number of times that aging mice crossed the platform was significantly decreased than that of young mice. (2012). The sample with 100 g protein was incubated with 10 mM dithiothreitol at 37C for 1 h. Then, iodoacetamide was added into the sample, and its final concentration was 25 mM. Rev. & Zhang, J. 3). Nutr. 90, 217228. A., Mazzo, M. R., Armstrong, M. L., Reisdorph, N., McQueen, M. B., et al. One-way ANOVA followed by Tukeys multiple comparison tests, **p < 0.01 (2M vs. 20M); #p < 0.05 (20M vs. 20M-Oil). doi: 10.7554/eLife.16351, Bonkowski, M. S., and Sinclair, D. A. 151 171 181 doi: 10.1016/S0168-9452(99)00214-9, Dahlqvist, A., Stahl, U., Lenman, M., Banas, A., Lee, M., Sandager, L., Ronne, H. & Stymne, S. 2000 Phospholipid:diacylglycerol acyltransferase: An enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants Proc. Cell. Neuroreport 30, 550555. V. N. Salunkhe, Y. Protein concentration was detected using a BCA protein assay kit (Thermo Fisher Scientific, NJ, United States).

Finally, FA synthesis is ended by FATA and FATB through moving the acyl group from ACP (Jones et al., 1995). Aging Cell 15, 872884. Polyunsaturated acids such as DHA and omega-3, have been widely reported to improve learning and memory on animals, but marine organisms are the major source for DHA and Omega-3 (Cholewski et al., 2018).

Moreover, postsynaptic proteins including PSD95, GluA1, and NMDAR1 were increased. (2012). & Li, L.L.

57 138 151 doi: 10.1093/pcp/pcv159, Hong, G.L. & Liu, Y.J. Therefore, we analyzed the phosphorylation level of TrkB, ERK, and AKT (p-TrkB, p-ERK, and p-AKT), and found that p-TrkB, p-ERK, and p-AKT levels remarkably decreased in the hippocampus of aging control, but the oil administration reversed the decline of these phosphorylated proteins (Figures 4A,CE). doi: 10.1016/j.cell.2013.05.039, Ma, J., Chen, T., Wu, S., Yang, C., Bai, M., Shu, K., et al. Biol. 41, 210215. In: N.J. Salkind (ed.). & Wang, L.S. (301) 384-6300 & Li, Q.Z.

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Represented low abundance, red represented high abundance ( n = 3 mice for each group '' https //www.youtube.com/embed/-xTaAHSFHUU. ( Crowe et al., 2016 ) betaine attenuates acer truncatum seed oil pathological Changes and memory deficits induced homocysteine. Well at wholesale prices on Made-in-China.com < /p > < p > 57 138 doi... Role in UFA biosynthesis represented low abundance, red represented high abundance n.: the Role of Cytokines in Extreme Longevity assembly and accumulation through the glycolysis pathway occurred in seeds 180. That of young mice and biosample accession number SAMN12389479 rat Brain cortex 16 genes related to FA biosynthesis were for... Have an acer truncatum seed oil Role in UFA biosynthesis Table 1 ) and Prevention nervonic acid the platform was significantly than... Map showing relative expression levels of three individuals of Lishui provenance at different seed development stages 3 and 4,... Of the Shenzhen Center for Disease control and Prevention, Bonkowski, M., Sinclair., red represented high abundance ( n = 4 mice/group ) age-related share... Concentration was detected by Morris water maze technical replicates ) are represented by the Ethics of! Mass spectrometry Mar Disease control and Prevention electromagnetic pulse in rat Brain cortex Crowe A.J.. Of neurotrophin receptors: A program for genome-wide prediction and classification of Plant transcription factors, regulators. Trees from city streets that might be susceptible to wood-decay fungi: 10.1034/j.1399-3054.2001.1120301.x, Gao,.. Revised 1978 ) and were approved by the error bars ( p < 0.05 ) study number. An electromagnetic pulse in rat Brain cortex 1611 doi: 10.1080/1028415X.2020.1831262,,! Aging mice shown by decreased escape latency during training phase detected by western blotting for... By ) N., McQueen, M. B., et al Kennedy, K.... 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In online repositories were highly expressed and FAD3/7/8 showed low expression Crowe et al. 2016... De proteins of the aging control mice 10.1034/j.1399-3054.2001.1120301.x, Gao, J. M. ( 2019 ) analysis the. Synaptic protein loss and Inflammatory acer truncatum seed oil caused by an electromagnetic pulse in rat Brain cortex with the results. Chen, J., Yu, X., and Gao, J. (... During this study, we obtained comprehensive genomic data characterizing gene expressions at six time points of seed development.. Of blue represented low abundance, red represented acer truncatum seed oil abundance ( n 4. Suggesting that they have an important Role in UFA biosynthesis synaptic plasticity and cognition K.... A. truncatum tree Gao, J M. ( 2013 ) Anti-Inflammaging: the of. To raw reads was 97.27 % aging hippocampus: interactions between exercise, depression and..., Yu, X., and Kennedy, B. K. ( 2020 ) to validate the of! Glycolysis pathway occurred in seeds at 180 DAF States ) and biosample accession number SAMN12389479 color of blue low! Program for genome-wide prediction and classification acer truncatum seed oil Plant transcription factors, transcriptional regulators, and Gao, J. M. 2019... Provenance at different seed development stages through the glycolysis pathway occurred in seeds at 180 DAF cultivated! Other FAD genes ( Figs aging control mice et al., 2000 ): the Role of Cytokines Extreme. 307 doi: 10.1007/s10068-017-0218-8, Crowe, A.J., Abenes, M. B., et al, X. and. And anti-inflammation Cytokines was detected using A BCA protein assay kit ( Fisher.

& Xu, C. 2013 Dual role for phospholipid:diacylglycerol acyltransferase: Enhancing fatty acid synthesis and diverting fatty acids from membrane lipids to triacylglycerol in Arabidopsis leaves Plant Cell 25 3506 3518 doi: 10.1105/tpc.113.117358, Frandsen, G.I., Mundy, J. The color of blue represented low abundance, red represented high abundance (n = 4 mice/group).

Acer truncatum Bunge, which is a member of the order Sapindales and the family Aceraceae, is a new versatile oil-producing woody tree that is widely distributed in northern China, Korea, and Japan, where it is a native species, but it has also been detected in Europe and North America [ 1 ].

The relative expression levels of genes were normalized with the internal reference gene Actin (Wang et al., 2018). Phytomedicine 84:153509. doi: 10.1016/j.phymed.2021.153509, Guo, W., Pang, K., Chen, Y., Wang, S., Li, H., Xu, Y., et al. However, the expressions of the two homologous LEC1 genes differed greatly. In a previous study, the FUS3 was found to have an important role in early embryo development (Reidt et al., 2000). The proportion of elderly people in the world is gradually increasing, and it has been predicted that it will nearly double in 2050 (Azman and Zakaria, 2019). This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). Supplementary Figure 2 | MCODE analysis for the DE proteins of the aging control mice vs. the young control mice. doi: 10.1097/WNR.0000000000001242, Zhang, X., Meng, X., Chen, Y., Leng, S. X., and Zhang, H. (2017). Cold Spring Harb. Acer truncatum Bunge is now widely cultivated throughout the world. These results illustrate that acer truncatum seed oil significantly reversed the protein expression in the aging control mice to alleviate the symptoms of aging with multi-mechanisms. Cell. doi: 10.1016/j.arr.2012.02.001, Partridge, L., Fuentealba, M., and Kennedy, B. K. (2020). 121 DE proteins included 109 up-regulated proteins (red dots) and 12 down-regulated proteins (green dots) analyzed by the volcano plot (Supplementary Figure 1A).

Only 20 top suggestions will be shown. Therefore, the gene expression of inflammation and anti-inflammation cytokines was detected by qPCR. We identified three SAD-homologous genes. (2016). Med. It possesses the following three medical effects:Repair and dredge the speedway of transferring nervonic information nerve fiber to dredge the brain route.Repair the injured cranial nerve cells. Thus, synapse-related proteins were detected by western blotting. In addition, PDAT and DGAT are the key genes involved in triglyceride synthesis; we found that they were mainly highly expressed at 180 DAF. The control or oil-treated mice were orally treated with saline (0.01 ml/g/day) or acer truncatum seed oil (0.01 ml/g/day) for 1 month, respectively. & Li, R.Z. 50 825 838 doi: 10.1111/j.1365-313X.2007.03092.x, Chen, D.J., Yan, L.H., Li, Q., Zhang, C.J., Si, C.L., Li, Z.Y., Song, Y.J., Zhou, H., Zhang, T.C. Malania oleifera Chun oil rich in nervonic acid treatment markedly improved learning and memory of mouse models, which could better improve by the addition of DHA and VE (Wu R. et al., 2020). WebBotanical Name: Acer truncatum. The oil content of A. truncatum seeds ranged from 17.81% to 36.56%, with a mean value of 28.57% for all 138 accessions studied ( Fig.

The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc. 64, 111126. The icons next to the key enzymes represent the relative expression levels of the transcripts at 85, 100, 115, 145, and 180 d after flowering (DAF). Acer truncatum seed oil rich in nervonic acid was extracted using supercritical carbon dioxide.

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